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. 2020 May 1;11(5):304. doi: 10.1038/s41419-020-2467-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a GSEA plot indicating that BECN1 expression is inversely correlated with JAK2/STAT3 enrichment gene signatures in the GEO database (GSE17536). b Western blot analysis of the indicated proteins in LoVo, HCT116 and SW48 cells expressing shRNA-NC or shRNA-BECN1. c Western blot analysis was used to determine the level of nuclear STAT3 and p-STAT3 in HCT116 cells stably expressing negative control, shRNA-BECN1#1 or shRNA-BECN1#2. d An immunofluorescence assay was performed to examine STAT3 localization in HCT116 cells among the indicated groups. Scale bar: 20 μm. e qPCR was used to examine the expression of IL-6 and VEGF-C in the indicated HCT116 cells. f STAT3 luciferase activity was measured in the indicated HCT116 cells transfected with PGL6-p-STAT3 and pRL-TK plasmids after 24 h of incubation by a dual-luciferase assay. The values are the mean ± SD for triplicate samples (*P < 0.05 by an independent Student’s t test).