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. 2020 May 1;11(5):304. doi: 10.1038/s41419-020-2467-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a 293T cells were cotransfected with HA-STAT3 and FLAG-BECN1 plasmids, and immunoprecipitation experiments were used to examine the interaction between HA-STAT3 and FLAG-BECN1. b, c Immunoprecipitation was performed to determine the interaction between BECN1 and STAT3 in both HCT116 and LoVo cells. d Immunofluorescence staining showed the endogenous interaction between BECN1 and STAT3 in the cytoplasm of HCT116 cells. Scale bar: 20 μm. e, f Immunoprecipitation was performed with a JAK2 antibody, and western blot assays were used to examine the immunoprecipitated complexes, including JAK2, BECN1, and STAT3, among the indicated groups.