Fig. 5. Cre recombination induced by blue light in vivo PA-Cre 3.0 mouse.
a Schematic representation of PA-Cre 3.0 mouse targeting strategy. PA-Cre 3.0 knocked-in to Gt(ROSA)26Sor (Rosa26) locus with CAG promoter and stop sequence added between FRT cassettes. Flp-mediated excision of the FRT-flanked stop cassette (Stop) induces PA-Cre 3.0 expression. b Representative bright field and tdTomato fluorescent images of mouse embryonic fibroblast cells derived from Rosa26PA-Cre B4/Ai14 mouse line illuminated with blue light. Diagram showed the experimental time course. The CAG-Flpe lentivirus was infected at the serial 2 days. The following day, the cells were illuminated for 24 h (470 ± 20 nm, 2 W/m2, 24 h continuous) (n = 3, Scale bar: 100 μm). c Representative liver images freshly isolated from Rosa26PA-Cre B4/Ai14 mice. CAG promoter-mediated Flp plasmids were transfected into PA-Cre 3.0 B4 mouse livers using HTV method. The mice were maintained in the dark, ambient light, or under blue-light illumination (470 ± 20 nm, 200 W/m2, 16 h continuous) (n = 3–4 mice/group, Scale bar: 1 mm).