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. 2020 May 1;11(5):302. doi: 10.1038/s41419-020-2506-0

Fig. 3. LINC01116 exerts its biological roles by regulating IL-1β in glioma.

Fig. 3

a Mean-centered, hierarchical clustering of 715 transcripts altered (≥2-fold change) in Ln229 cells transfected with si-NC or si-LINC01116, with three repeats. b Gene ontology analysis for all genes with altered expression after knockdown of LINC01116. c Relative expression levels of the selected 6 mRNAs in Ln229 and U87 cells transfected with si-NC or si-LINC01116 were measured using qRT-PCR. d–e IL-1β protein levels were detected by western blot and ELISA analysis in Ln229 and U87 cells transfected with si-NC and si-LINC01116. f Quantification of CCK8 assay was performed on Ln229 and U87 cells proliferation, which were cultured with or without IL-1β (10 ng/ml) 24 h later. g The promotion of Ln229 and U87 cells proliferation by overexpression of LINC01116 was significantly reversed by neutralizing IL-1β (1 ng/ml). Error bars represent mean ± SD.*P < 0.05, **P < 0.01.