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. 2020 May 1;11(5):302. doi: 10.1038/s41419-020-2506-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a ChIP-qPCR of DDX5 of the promoter region of IL-1β loci after knockdown of LINC01116 in glioma cells. Antibody enrichment was quantified relative to the amount of input DNA. IgG was used as the control. b–d qRT-PCR, ELISA, and western blot assays were used to detect the expression of IL-1β in Ln229 and U87 cells transfected with si-NC or si-DDX5. e–g The promotion of IL-1β (mRNA and protein) by overexpression LINC01116 was significantly reversed by knockdown of DDX5 in Ln229 and U251 cells, based on qRT-PCR, ELISA, and western blot assays. h Illustrative model showing the proposed mechanism of LINC01116 in glioma cell. Error bars represent mean ± SD. *P < 0.05, **P < 0.01.