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. 2020 May 1;11:2148. doi: 10.1038/s41467-020-15978-3

Fig. 1. MD2-TLR4 complex activation in hearts of diabetic mice.

Fig. 1

A mouse model of type 1 diabetes mellitus was developed by administering streptozotocin to C57BL/6 mice. Heart tissues were harvested at 16 weeks [Con = non-diabetic controls, STZ = diabetic mice]. a Representative immunoblot for MD2 and TLR4 in mouse cardiac tissue. GAPDH was used as loading control. Densitometric quantification of blots showing MD2 (white bars) and TLR4 (black bars) [n = 4; 3 Con and 3 STZ samples shown in immunoblots; means ± SEM]. b Representative immunoblots showing co-immunoprecipitation of TLR4 and MD2 in mouse heart tissues at 16 weeks following onset of diabetes [IP = precipitating antibody, IB = immunoblot antibody; n = 4; 2 Con and 2 STZ samples shown in immunoblots]. c Representative immunofluorescence staining of mouse heart tissues at 16 weeks for MD2 (red), macrophage marker F4/80 (green), and myocyte marker α-actin (green). Slides were counterstained with DAPI (blue) [n = 4]. Source data are provided as a Source Data file.