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. 2020 May 1;11:2165. doi: 10.1038/s41467-020-15982-7

Fig. 3. Abcc8 mediates hypoglycemia-induced inhibition GE-ERαvlVMH neurons.

Fig. 3

a Relative mRNA levels of Abcc8 in female GI- and GE-ERαvlVMH neurons measured by real-time RT-qPCR. N = 7 or 8 neurons from three mice per group. ***P < 0.0001 in two-sided t-tests. b KATP current in a female GE-ERαvlVMH neuron in response to glucose fluctuations with or without tolbutamide (200 µM). c Hypoglycemia-induced KATP currents in female GE- and GI-ERαvlVMH neurons. N = 12 or 14 neurons from three mice per group. ***P < 0.0001 in two-sided t-tests. d, e Hypoglycemia-induced changes in resting membrane potential in female GE- d or GI-ERαvlVMH neurons e in the absence or the presence of tolbutamide (200 µM). N = 10 or 13 neurons from three mice per group. ***P < 0.0001 in two-sided t-test. f CRISPR-mediated knockout of Abcc8 in one side of ERαvlVMH neurons leaving the other side as controls in the same female mice. g The composition (%) of GE, GI, and no glucose-sensing neurons from ERαvlVMH populations in the control and Abcc8-knockout sides. N = 19 or 20 neurons from two mice per group. h KATP currents measured in the control and Abcc8-knockout sides of female ERαvlVMH neurons in the presence of 1 mM glucose. Note: since there were no GE neurons in the knockout side, data from knockout side were collected from nonGI neurons g, while data for control side were collected from identified GE neurons. N = 8 or 10 neurons from two mice per group. ***P < 0.0001 in two-sided t-tests. i CRISPR-mediated knockout of both Ano4 and Abcc8 in both sides of ERαvlVMH neurons in Esr1-Cre female mice, while WT mice were used as controls. j–l Effects of icv saline or 2-DG on blood glucose in control j or in mice with both Ano4 and Abcc8 disrupted in ERαvlVMH neurons k. N = 6 or 9 mice per group. *P = 0.0185, **P = 0.0053, at 30 min ***P < 0.0001 and at 60 min ***P = 0.0007 in two-way ANOVA analysis followed by post hoc Sidak tests. l Area under the curve for data in j and k. N = 6 or 9 mice per group. In WT *P = 0.0139, in Esr1-Cre *P = 0.0104, and **P = 0.0036 in two-sided t-tests. Results in a, c–e, h, j–l are shown as mean ± SEM. Source data are provided as a Source Data Fig. 3.