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. 2020 May 1;11:2163. doi: 10.1038/s41467-020-15729-4

Fig. 3. AsNRs can drive customized programs after being activated.

Fig. 3

a Experimental strategy to test the effectiveness of customized program activation. The RFP reporter gene is promoted by TRE and the reporter is activated after tTA is released from the membrane. Colored diamonds represent receiver cells in different states: green, RFP reporter that is not activated; orange, RFP reporter that is activated. b Analysis of receiver cells after sorting by flow cytometry showing that receiver cells did not activate the RFP reporter. c Image of fluorescence showed that the RFP reporter in the receiver cells was not activated without contact with the sender cells. d After adding HUVECs (sender cells), the receiver cells turned red within 24 h after cell–cell contact. e Quantification of the receiver cells showed that the number of RFP + receiver cells gradually increased within 24 h. Scale bars = 50 μm in c and d. Error bars: SEM. Significance: *p < 0.05 (n = 3 samples per group in e).