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. 2020 May 1;11:2163. doi: 10.1038/s41467-020-15729-4

Fig. 5. Engineered cells with AsNRs can sense the sprouting vessels of tumors in adults.

Fig. 5

a Schematic showing the experimental strategy. Tissue and blood were collected at 24 h after engineered cell injection and engineered cells turned red after they sensed sprouting vessels. b Whole-mount fluorescence images of tissues showing that engineered T cells failed to turn red in the hearts, livers, spleens, or lungs, but robust RFP signals were detected in tumors, indicating that the AsNRs were specific for Apj in vivo. c CD4+ and CD8+ T cells from blood were sorted and the proportion of RFP-positive cells increased significantly at D7 compared with those at D0 and D21. d The proportion of RFP+ cells increased at D21, indicating that engineered cells were retargeted to tumors as tumors grew. e Proportion of RFP+ cells in blood and tumors at D0, D7, and D21. (blood: n = 3 samples; tumor: n = 5 mice per group). Scale bars = 1 mm in b. Error bars: SEM. Significance: *p < 0.05.