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. 2020 Mar 30;17:871–893. doi: 10.1016/j.omtm.2020.03.028

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© 2020 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Brain Histology and Immunohistochemistry for Mouse 758

(A) H&E-stained section of brain from mouse 758. Arrow points to needle inoculation site. Immediately past the needle tract is a tumor showing extensive necrosis that is attributed to rQNestin34.5v.2 injection. (B) HSV immunohistochemistry (IHC) reveals extensive tumor infection with extension of the infection toward the ventricle (arrow). (C) High-power microphotograph of HSV IHC, showing cells at the ependymal surface and subependymal region that are HSV antigen-positive. (D) CD45 IHC showing several inflammatory cells present along the ependyma. (E) IHC for nestin in reactive astrocytes adjacent to tumor. Arrow points to a mitotic astrocyte. (F) Ependyma showing mild, yet extensive, nestin immunohistochemical staining in the same areas where HSV and CD45 cells are observed.