Table 2.
Histopathology and Immunohistochemistry Evaluation
| Animal ID | Day of Death | CD45 Immunoreactive | Interpretation | ||
|---|---|---|---|---|---|
| Group 1 | |||||
| Control males, scheduled deaths | 101 | 4 | no significant microscopic lesions | ||
| 103 | 4 | ||||
| 105 | 4 | ||||
| 108 | 31 | ||||
| 110 | 31 | ||||
| 112 | 31 | ||||
| 113 | 61 | ||||
| 116 | 61 | ||||
| 117 | 61 | ||||
| Control females, scheduled deaths | 151 | 4 | no significant microscopic lesions | ||
| 153 | 4 | ||||
| 155 | 4 | ||||
| 157 | 31 | ||||
| 160 | 31 | ||||
| 162 | 31 | ||||
| 163 | 61 | ||||
| 165 | 61 | ||||
| 167 | 61 | ||||
| Group 2 | |||||
| CPA males, scheduled deaths | 202 | 4 | no significant microscopic lesions | ||
| 204 | 4 | ||||
| 206 | 4 | ||||
| 208 | 31 | ||||
| 210 | 31 | ||||
| 211 | 31 | ||||
| 214 | 61 | ||||
| CPA males, early deaths | 215 | 52 (FD) | cause of death not apparent | ||
| 218 | 55 (FD) | cause of death not apparent | |||
| CPA females, scheduled deaths | 252 | 4 | no significant microscopic lesions | ||
| 254 | 4 | ||||
| 256 | 4 | ||||
| 257 | 31 | ||||
| 259 | 31 | ||||
| 262 | 31 | ||||
| 267 | 61 | ||||
| CPA females, early deaths | 264 | 59 (FD) | cause of death not apparent | ||
| 265 | 54 (FD) | cause of death not apparent | |||
| Group 3 | |||||
| 4.2 × 104 PFU virus, male scheduled deaths | 302 | 4 | minimal HSV infection of nervous tissue in the absence of cytopathic effect or inflammatory change | ||
| 304 | 4 | no significant microscopic lesions | |||
| 306 | 4 | √ | minimal HSV infection of nervous tissue in the absence of cytopathic effect or inflammatory change | ||
| 307 | 31 | no significant microscopic lesions | |||
| 312 | 31 | no significant microscopic lesions | |||
| 315 | 31 | no significant microscopic lesions | |||
| 314 | 61 | no significant microscopic lesions | |||
| 317 | 61 | no significant microscopic lesions | |||
| 4.2 × 104 PFU virus, female scheduled deaths | 352 | 4 | no significant microscopic lesions | ||
| 354 | 4 | √ | minimal viral infection with no cytopathic effect and a minimal inflammatory response | ||
| 356 | 4 | √ | minimal viral infection of ependyma and periventricular tissues in the absence of cytopathic effect and a minimal inflammatory response | ||
| 358 | 31 | no significant microscopic lesions | |||
| 359 | 31 | no significant microscopic lesions | |||
| 361 | 31 | no significant microscopic lesions | |||
| 367 | 61 | no significant microscopic lesions | |||
| 4.2 × 104 PFU virus, male early deaths | 309 | 6 (FD) | cause of death not apparent | ||
| 316 | 53 (FD) | cause of death not apparent | |||
| 4.2 × 104 PFU virus, female early deaths | 365 | 54 (FD) | cause of death not apparent | ||
| 364 | 55 (FD) | √ | death attributed to viral infection in brain | ||
| Group 4 | |||||
| 3 × 103 PFU virus + CPA, male scheduled deaths | 402 | 4 | no significant microscopic lesions | ||
| 404 | 4 | no significant microscopic lesions | |||
| 406 | 4 | there was a single HSV antigen-positive cell that was not associated with cytopathic effect or an inflammatory response | |||
| 408 | 31 | no significant microscopic lesions | |||
| 410 | 31 | no significant microscopic lesions | |||
| 411 | 31 | no significant microscopic lesions | |||
| 415 | 61 | no significant microscopic lesions | |||
| 3 × 103 PFU virus + CPA, female scheduled deaths | 452 | 4 | no significant microscopic lesions | ||
| 454 | 4 | no significant microscopic lesions | |||
| 456 | 4 | no significant microscopic lesions | |||
| 458 | 31 | minimal focal viral infection in the absence of inflammatory responses or cytopathic effect | |||
| 459 | 31 | no significant microscopic lesions | |||
| 461 | 31 | no significant microscopic lesions | |||
| 464 | 61 | no significant microscopic lesions | |||
| 466 | 61 | no significant microscopic lesions | |||
| 3 × 103 PFU virus + CPA, male early deaths | 418 | 53 (FD) | cause of death not apparent | ||
| 413 | 54 (FD) | cause of death not apparent | |||
| 3 × 103 PFU virus + CPA, female early death | 467 | 53 (FD) | cause of death not apparent | ||
FD, found dead.