Table 3.

Number of unique subclones that have a different codon sequence at the location of the N-gly site to that of the major clone

Patient	Disease event	No. of unique tumor-related subclones	No of unique tumor subclones with different codon sequence in N-gly site region to the MC (% of total subclones)	Subclones with different codon sequence in N-gly region
				Percentage of subclones without N-gly site	Percentage of subclones with N-gly site
1	Diagnosis	2727	107 (3.92)	1.69	2.24
	Transformation	1690	110 (6.51)	1.83	4.67
2	First relapse	3530	96 (2.72), 113 (3.20), 2062 (58.41), 226 (6.40)	0.99, 0.99, 1.13, 2.78	1.73, 2.21, 57.28, 3.63
	Third relapse	3032	145 (4.78), 239 (7.88), 982 (32.39), 737 (24.31)	0.69, 3.10, 1.25, 18.17	4.09, 4.68, 31.13, 6.13
	Transformation	3966	134 (3.38), 148 (3.73), 1370 (34.54), 747 (18.84)	1.46, 1.84, 2.29, 15.05	1.92, 1.89, 32.25, 3.78
3	Second relapse	4103	385 (9.38)	2.53	6.85
	Third relapse	2057	179 (8.70)	2.09	6.61
	Transformation	1592	455 (28.58)	1.82	26.76
4	First relapse	81	28 (34.57), 0	2.47, 0	32.1, 0
	Second relapse	248	14 (5.65), 1 (0.40)	2.02, 0.40	3.63, 0
5	First relapse	140	22 (15.71)	2.86	12.86
	Third relapse	371	21 (5.66)	0.81	4.85
6	FL diagnosis	2510	946 (37.69)	1.59	36.10
	tFL diagnosis	3442	265 (7.70)	2.06	5.64
	tFL relapse	4749	214 (4.51)	2.70	1.81

The percentage of these subclones that are either motif positive or motif negative are provided. Percentages were calculated from the total number of unique subclones identified. Values were rounded to 2 decimal points.