Table 4.

N-gly site status in most and least diverse subclone based on degree of SHM compared with V gene germline sequence

	Patient 1		Patient 2			Patient 3			Patient 4		Patient 5		Patient 6
Least mutated subclone	FL	tFL	FL1	FL3	tFL	FL2	FL3	tFL	FL1	FL2	FL1	FL3	FL	tFL	tFL1
Percentage homology to G.L V gene sequence	96.4	98.0	88.7	88.3	94.4	84.7	86.1	95.6	89.4	89.0	98.0	89.1	97.8	93.0	92.3
N-gly site presence (Y/N)	Y	Y	Y, Y, Y, Y	Y, Y, Y, N	N, N, N, Y	Y	Y	N	Y,Y	Y, Y	N	Y (FL1 site)	Y	Y	Y
Most mutated subclone
Percentage homology to G.L V gene sequence	84.3	83.9	84.7	84.3	84.3	79.0	79.4	79.6	87.4	86.9	87.5	81.5	79.4	71.3	73.5
N-gly site presence (Y/N)	Y	Y	Y, Y, Y, Y	Y, Y, Y, Y	Y, Y, Y, Y	Y	Y	Y	Y,Y	Y,Y	Y	Y	Y	Y	Y
Percentage difference in homology between least and most mutated subclones	12.1	14.1	4.0	4.0	10.1	5.7	6.7	16.0	2.0	2.1	10.5	7.6	18.4	21.7	18.8

Sequences were analyzed for presence or absence of the N-gly motif site or sites found in the major clone.