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. 2020 Apr 18;23(5):101068. doi: 10.1016/j.isci.2020.101068

Figure 2.

Figure 2

Cholesterol-Mediated Immunity Primarily Acts through a p38/PMK-1 MAPK Pathway

(A) Venn diagram showing upregulated genes (20 versus 5 μg/mL cholesterol) and downregulated genes (0 versus 5 μg/mL cholesterol).

(B) Gene ontology analysis of upregulated and downregulated genes in animals grown on 20 and 0 μg/mL cholesterol, respectively. The cutoff is based on the filtering thresholds of P < 0.05 and arranged according to the representation factor.

(C) Gene ontology analysis of shared genes between animals grown at 20 versus 5 μg/mL and 0 versus 5 μg/mL cholesterol. The cutoff is based on the filtering thresholds of P < 0.05 and arranged according to representation factor.

(D) Representation factors of immune pathways for the upregulated and downregulated immune genes in animals grown at 20 versus 5 and 0 versus 5 μg/mL cholesterol, respectively.

(E) WT and pmk-1(km25) animals were grown on 20 and 5 μg/mL cholesterol, exposed to P. aeruginosa, and scored for survival. WT animals grown on 5 μg/mL cholesterol (control) versus 20 μg/mL, P < 0.0001; pmk-1(km25) 5 μg/mL, P < 0.0001; pmk-1(km25) 20 μg/mL, P > 0.0001. pmk-1(km25) 0 μg/mL versus pmk-1(km25) 5 μg/mL, P = NS.

(F) WT and daf-16(mu86) animals were grown on 20 and 5 μg/mL cholesterol, exposed to P. aeruginosa, and scored for survival. WT animals grown on 5 μg/mL cholesterol (control) versus 20 μg/mL, P < 0.0001; daf-16(mu86) 20 μg/mL, P = NS; daf-16(mu86) 5 μg/mL, P = NS.

(G) WT and skn-1(zj15) animals were grown on 20 and 5 μg/mL cholesterol, exposed to P. aeruginosa, and scored for survival. skn-1(zj15) animals grown on 5 μg/mL cholesterol (control) versus 20 μg/mL, P < 0.001.