Fig. 2.

Rb1 or Rb2 promote myogenic differentiation through activating a promyogenic kinase, Akt. (A) Rb1-treated C2C12 cells were differentiated for 2 days, followed by MHC immunostaining. (B) The MHC-positive myocytes shown in panel (A) were quantified as the number of nuclei per myotube. The values are represented as means ± SD of three independent experiments. **P < 0.01 compared with the control group. (C) Cell lysates from panel (A) were subjected to immunoblotting with antibodies to MHC, MyoD, and myogenin, phosphorylated Akt, total Akt, and pan-Cadherin used as a loading control. (D) Rb2-treated C2C12 cells were differentiated for 2 days, followed by immunoblotting against antibodies of MHC, MyoD, myogenin, phosphorylated Akt, total Akt, and pan-Cadherin used as a loading control. (E) Cells from panel (D) were immunostained for MHC expression (red) and with DAPI to stain nuclei (blue) to reveal myotube formation. (F) The MHC-positive myocytes shown in panel (E) were quantified as the number of nuclei per myotube. **P < 0.01 compared with the control group.

MHC, myosin heavy chain; SD, standard deviation.