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. 2020 Apr 23;13:3467–3476. doi: 10.2147/OTT.S243632

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© 2020 Li et al.

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ATF3 modulated transcription of ATRL4C. (A) The expression of ATF3 was positively correlated with ATRL4C in the TCGA database. (B) The expression of ATF3 and ARL4C were reduced in TNBC cell lines than the luminal cells. (C) Overexpression of ATF3 promoted ARL4C protein expression. (D and E) ATF3 stimulated ARL4C mRNA expression in two cell lines.(F) Chromatin immunoprecipitation-qPCR of the promoter region of ARL4C locus in two cell lines. Antibody enrichment was quantified relative to the amount of input DNA. An antibody against IgG was used as a negative control. (G) Dual-luciferase activity was detected in HEK293T cells co-transfected with pLVX-ATF3 or empty vector (pLVX) with pGL3-Control or pGL3-ARL4C reporter. **P < 0.01, ***P < 0.001 for t-test, n ≥ 3.

Abbreviations: GAPHD, glyceraldehydes-3-phosphate dehydrogenase; TNBC, triple negative breast cancer; pLVX, pLVX puro; IgG, immunoglobin G; TCGA, The Cancer Genome Atlas.