Fig. 2.

Decreased expression of MITF pathway genes induced by KRG. B16/F10 cells were seeded in 6-well plates, treated with the indicated concentrations of KRG, and then stimulated with α-MSH (10μM). Subsequently, RNA and proteins were extracted and MITF, TYR, TRP-1, and TRP-2 expression was measured by Quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR) (A) and western blotting (B). GAPDH was used as the internal control qRT-PCR and β-actin was used as the internal control for western blotting. ***p < 0.001 and **p < 0.05 were considered as statistically significant against the α-MSH-treated group only.

KRG, Korean Red Ginseng; α-MSH, α-melanocyte–stimulating hormone; MITF, microphthalmia-associated factor; TYR, tyrosinase; TRP-1, tyrosinase-related protein 1; TRP-2, tyrosinase-related protein 2.