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. 2020 Apr 24;26:e921877-1–e921877-11. doi: 10.12659/MSM.921877

Figure 3.

Figure 3

MiR-328-3p alleviates ox-LDL induced HUVECs injury. HUVECs were transfected with miR-328-3p or miR-NC prior to ox-LDL treatment. (A) qRT-PCR analysis of miR-328-3p expression. HUVECs were treated with 100 μg/mL ox-LDL for 24 hours. (B) Cell viability was detected using CCK-8 assay. (C) Flow cytometry was used to test cell apoptotic rate. (D) The expression of C-caspase 3 was examined by western blot. (E, F) The migration and invasion abilities were analyzed using Transwell assay. (G) The expression of Beclin, LC3-I, and LC3-II was measured using western blot. (H–J) The levels of TNF-α, IL-1β, and IL-10 were examined by ELISA assay. * P<0.05. HUVECs – human umbilical vein endothelial cells; ox-LDL – oxidized low-density lipoprotein; qRT-PCR – quantitative real-time polymerase chain reaction; CCK-8 – Cell Counting Kit-8; TNF – tumor necrosis factor; IL – interleukin; ELISA – enzyme-linked immunosorbent assay.