Skip to main content
. 2020 Apr 24;26:e921877-1–e921877-11. doi: 10.12659/MSM.921877

Figure 5.

Figure 5

FOXO4 is a target of miR-328-3p in HUVECs. (A) The putative binding sites between miR-328-3p and FOXO4 were predicted. (B, C) Dual-luciferase reporter assay in HUVECs after the co-transfection with FOXO4-wt or FOXO4-mut and miR-195-5p mimic or miR-NC or anti-miR-NC or anti-miR-328-3p was conducted. (D) RIP assays were carried out to confirm the interaction of miR-328-3p and FOXO4 to Ago2 in HUVECs. (E) FOXO4 expression was detected using western blot in HUVECs transfected with miR-328-3p or anti-miR-328-3p or the corresponding negative controls. * P<0.05. FOXO4 – forkhead box protein; HUVECs – human umbilical vein endothelial cells; RIP – RNA immunoprecipitation.