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Preventive EA decreased D-gal-induced tau hyperphosphorylation levels and preserved synapse-associated proteins. The protein levels of tau-5, PHF-1, PSD95, and synapsin-1 in the hippocampus were detected using western blotting (a, b). Comparison of the relative expression of tau-5, PHF-1, PSD95, and SYN in the hippocampus (c,d,e,f). GAPDH was used as a loading control. The data were expressed as mean ± SD (n = 6). ∗P < 0.01 vs. model; #P < 0.01 vs. sham EA.
