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. 2020 Apr 23;2020:9473497. doi: 10.1155/2020/9473497

Figure 3.

Figure 3

The difference in the effects of CLEC5A knockdown on NLRP3-inflammasome expression in THP-1 cells between AOSD patients and healthy controls. (a) CLEC5A expression in THP-1 cells was knocked down by CLEC5A-specific siRNA (Dharmafect) and a nonspecific siRNA (negative control) for 48 hours. (b) Cells were stimulated with 10% plasma from AOSD patients (n = 6) and HC (n = 4) for 6 h at 37°C, and cell lysates were harvested for the determination of (c) NLRP3, (d) caspase-1, (e) IL-1β, and (f) IL-18 by Western blotting. α-Tubulin is used as loading control. AOSD: adult-onset Still's disease; HC: healthy controls; IL: interleukin. Bars and error bars indicate mean and standard deviation, respectively. The p values were determined by Bonferroni's posttest.