Fig. 6.

Example of the output to be achieved with an extended system. Bright blue: seed pixels, bright green: radicle pixels, bright red: haustorium pixels; dark blue bounding box: seed objects, dark green bounding box: radicle objects, dark red bounding box: haustorium objects. The input image contains 6 seeds, 3 of which are germinated and 2 that have developed haustoria. The extended version of DiSCount would likely compute a germination rate of 3/6 = 0.50 and haustoria development rate of 2/3 = 0.67. In this example, considering that the extended version detects 400 seed pixels, 300 radicle pixels and 100 haustoria pixels, the result would be mean individual object sizes of 400/6, 300/3 and 100/2, respectively. Considering that the camera characteristics are such that 1 pixel equals to 0.1 mm², these would result in mean individual sizes of 6.67 mm² (seeds), 10 mm² (radicles) and 5 mm² (haustoria)