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. 2020 May 1;18:81. doi: 10.1186/s12957-020-01850-7

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Microarray analyses reveal a role of SeNPs in microRNA expressions. a The Venn diagrams depict the number of genes regulated by SeNP treatment in LnCaP and LnCaP-A cells identified by microarray analysis. Overlapped regions represent the number of genes regulated by SeNP treatment in both LnCaP and LnCaP-A cells. b Heatmap clustering analysis of microRNA expression profile of the overlapped targets in a in both LnCaP and LnCaP-A cells. Each column represents the microRNAs expression profile. c Fold change of top differentially expressed genes by SeNP treatment. d, e Confirmation of miR-16 expression in d LNCaP and e LNCaP-A after SeNP treatment by qRT-PCR analysis