Fig. 2.

Inhibition of Prdx1 restored primary cilia and suppressed tumor invasion. a Transfection efficiency of lentivirus in EC9706 cells. EC9706 cells were transfected with plasmids expressing shRNA or OE-RNA and green fluorescent protein. Green fluorescent protein was detected as a marker of transfection efficiency by fluorescence microscopy (100×). b Western blotting was used to analyze the expression of Prdx1 in each group. GAPDH served as a loading control. Full-length blots/gels are presented in Supplementary Fig. X. c Analysis of western blot signals using Image J. The relative protein levels are indicated as bars. All experiments were performed in triplicate and results are expressed as mean ± SEM. d Immunofluorescence was used to detect the changes of the cilium marker protein (red: acetyl-α-tubulin, blue: DAPI, white arrows: cilia). e Number of cells with cilia (%) in each group. f Transwell invasion experiment to detect the changes in cell invasion capacity. G Number of invasion cells in each group. *P < 0.05, **P < 0.01, ***P < 0.001