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. 2020 May 1;20:372. doi: 10.1186/s12885-020-06898-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Tripolin A reversed the promotion effect of over-expressed Prdx1 on p-Aurora A and HDAC6. a Western-blot was used to detect changes of Aurora A expression in EC9706 cells treated with different concentrations (0, 1.5, 4.5, 7.5, and 10.5 μmol/L) of Tripolin A. b Relative gray level for p-Aurora A/Aurora A in cells after treatment with different concentrations of Tripolin A. c Changes of p-Aurora A/Aurora A expression in EC9706 cells treated with 4.5 μmol/L Tripolin A for different lengths of time (0, 2, 4, 8, and 12 h). d Relative gray level of p-Aurora A/Aurora A in cells after treatment with 4.5 μmol/L Tripolin A for different lengths of time. e The expression of NEDD9, p-Aurora A, Aurora A, and HDAC6 after EC9706 cells treated with 4.5 μmol/L Tripolin A for 2 h. f Analysis of western blot signals using Image J. GAPDH was used as a loading control. g The expression of NEDD9, p-Aurora A, Aurora A, and HDAC6 in different treatment groups. The OE-Prdx1 group was EC9706 cells transfected with the OE-Prdx1 lentivirus, and then treated with 4.5 μmol/L Tripolin A for 2 h. h Relative expression of NEDD9, p-Aurora A and HDAC6 in cells of different groups. GAPDH was used as a loading control. Full-length blots/gels are presented in Supplementary Fig. X. *P < 0.05, **P < 0.01, ***P < 0.001