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. 2020 May 1;11:168. doi: 10.1186/s13287-020-01680-0

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — AD-MSC-conditioned medium administration improves irrigation of peripheral nerves in diabetic mice. a Representative confocal images of longitudinal sections of sciatic nerves obtained from 26-week-old non-diabetic and diabetic mice treated with vehicle, conditioned medium derived from non-preconditioned AD-MSCs, or conditioned medium derived from DFX-preconditioned AD-MSCs. Blood vessels were stained with BS-1Lectin. b Quantification of vascular area in sciatic nerves. c Representative bright-field images of gastrocnemius muscle sections stained with hematoxylin and eosin for blood vessel detection. Arrows indicate blood vessels. d Quantification of blood vessel density in gastrocnemius muscle. Data are presented as number of blood vessels per muscle fiber. Quantitative data are presented as mean ± S.E.M. (n = 6, one-way ANOVA with Tukey post-test, *p < 0.05)