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. Author manuscript; available in PMC: 2020 May 2.
Published in final edited form as: Mol Cell. 2019 Sep 12;76(3):412–422.e5. doi: 10.1016/j.molcel.2019.08.015

Figure 4. Deletion of RBRs in CTCF Disturb Its Chromatin Binding.

Figure 4.

(A) CTCF ChIP-seq for WT (gray), ZF1Δ (black), and ZF10Δ (red) rescue cell lines. Heatmaps were generated by centering and rank-ordering on CTCF-binding sites. Those lost for ZF1Δ (top) or ZF10Δ (bottom) are shown.

(B) De novo motif discovery was called for binding sites in (A), and a black box encloses the eighth position in which A to G was specifically preferred by ZF1Δ.

(C) Boxplot showing the motif affinity scores for CTCF-binding sites in (A) compared with unchanged sites (Mann-Whitney test, p < 0.0001).

(D) Bar graph representing the top three genomic regions for CTCF sites in (A).

(E and F) Mean expression levels for differentially expressed gene Cdkn2a (E) and corresponding ChIP-seq tracks (F) under each condition.