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. Author manuscript; available in PMC: 2020 May 2.
Published in final edited form as: Antiviral Res. 2017 Aug 24;146:112–120. doi: 10.1016/j.antiviral.2017.08.014

Table 1.

MAb-mediated neutralization of tecVLPs in SW-13 cells.

MAb
 8A1
 11E7
 30F7
tecVLP EC50 (μg/mL) 95% Confidence interval Maximal RLU inhibition (%) EC50 (μg/mL) 95% Confidence interval Maximal RLU inhibition (%) EC50 (μg/mL) 95% Confidence interval Maximal RLU inhibition (%)
IbAr10200 0.046 0.031–0.070 99.639 ± 0.033 0.499 0.089–2.798 83.978 ± 0.329 0.777 0.451–1.34 98.682 ± 0.224
Sudan Al-Fulah 1.303 0.653–2.601 94.452 ± 0.184 0.572 2.080e(−4)–1575 53.108 ± 3.329 1.358 0.362–5.096 66.772 ± 4.409
Turky 1.412 0.948–2.104 92.766 ± 0.110 >1000 Ambiguous 71.948 ± 0.603 0.897 0.284–2.834 79.023 ± 1.372
Hoti 1.043 0.4473–2.433 96.343 ± 0.143 >1000 Ambiguous 52.368 ± 2.003 0.629 0.299–1.321 72.346 ± 3.639
Oman 0.780 0.595–1.022 87.925 ± 0.421 >1000 Ambiguous 43.052 ± 1.720 1.320 0.611–2.855 72.368 ± 1.604
SPU18/88 1.082 0.449–2.606 99.735 ± 0.029 0.137 0.06593–0.2858 95.563 ± 0.183 1.124 0.574–2.203 95.559 ± 0.700
ArD15786 2.057 0.187–22.67 99.670 ± 0.060 1.285 0.1707–9.668 80.706 ± 1.148 1.055 0.336–3.316 95.361 ± 0.280
YL04057 0.124 0.092–0.168 99.774 ± 0.022 >1000 Ambiguous 71.599 ± 1.688 0.403 0.273–0.595 99.368 ± 0.089
Afg09 0.668 0.521–0.857 94.711 ± 0.140 >1000 Ambiguous 69.726 ± 0.264 1.785 0.547–5.828 81.615 ± 2.091
NIV112143 1.410 0.607–3.280 92.205 ± 0.508 >1000 Ambiguous 41.826 ± 1.838 >1000 Ambiguous 52.170 ± 1.685
Baghdad-12 0.534 0.175–1.628 94.734 ± 1.603 0.036 0.020–0.063 99.657 ± 0.164 1.267 0.328–4.889 97.114 ± 1.631

MAbs 8A1, 11E7, and 30F7 were diluted in a 2-fold dilution series from 101 to 8 × 10−2 αg/mL and incubated with tecVLPs. Percent inhibition was calculated by comparing signal from tecVLP incubated with MAbs to mock-treated tecVLP signal. The data were used to fit a 4-paramenter equation to semilog plots of the concentration-response data and obtain an effective concentration 50% (EC50) for each monoclonal antibody against each tecVLP moiety. The EC50 values and 95% confidence intervals are shown as well as maximal inhibition. Maximal inhibition is shown as standard error of the a mean (n = 4).