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Complementation of heterologous NADPH-generating GapDHs to GapA deficiency in E. coli. (A) Overview of glycolytic pathways in E. coli. (B) Growth restoration of heterologous GapDHs in ΔgapA strain W3CG in minimal medium with glucose as the sole substrate. Error bars represent standard deviations of three replicates. n = 3. Ec, Escherichia coli; Sm, Streptococcus mutans; Ca, Clostridium acetobutylicum; Bs, Bacillus subtilis.
