Figure 2.
Inactivation of Nr4a2 in lactotropes of the mouse pituitary results in a significant decrease in Prl gene expression. Comparison of Nr4a2fl/fl/Prl-Cre (KO) and Nr4a2fl/fl (control) mice pituitary mRNAs. Targeted RT-qPCR validates selective loss of Prl mRNA in the Nr4a2 knockout mice. mRNA levels are shown for Pou1f1 and for 2 landmark genes for the somatotrope (Gh and Ghrh) and lactotrope (Prl and Drd2) lineages. The only comparison with a significant difference was for Prl (n = 3 mice). KO, knockout; mRNA, messenger ribonucleic acid; RT-qPCR, real-time quantitative polymerase chain reaction.