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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: J Immunol. 2019 Nov 27;204(1):101–111. doi: 10.4049/jimmunol.1800748

Fig. 2. Pneumolysin is sufficient to induce PMN transepithelial migration in a dose dependent manner.

Fig. 2.

(A) Apical sides of H292 monolayers on transwells were infected with 1x107 wild type B. subtilis or B. subtilis expressing ply. After infection, transepithelial migration of 1x106 human PMNs added to the basolateral side was quantified by MPO assay. fMLP and buffer alone were used as positive and negative controls, respectively. Asterisks indicate migration is significantly (**** = p<0.0001) greater than migration induced by the parental B. subtilis strain, as determined by one-way ANOVA, Post-hoc: Tukey. Shown is a representative of two independent experiments where each condition was tested in triplicate per experiment. (B) Apical sides of H292 monolayers were treated with indicated units of recombinant PLY (PLY). Transepithelial migration of 1x106 PMNs added to the basolateral side post-infection was quantified. fMLP and buffer alone were used as positive and negative controls, respectively. Shown is one representative experiment of four independent experiments where each condition was tested in triplicate per experiment. Asterisks indicate migration is significantly (** = p<0.005, **** = p<0.0001) greater than migration induced by buffer alone, as determined by one-way ANOVA, Post-hoc: Dunnett.