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. Author manuscript; available in PMC: 2021 Jul 1.
Published in final edited form as: Biochim Biophys Acta Gen Subj. 2020 Mar 12;1864(7):129595. doi: 10.1016/j.bbagen.2020.129595

Figure 4.

Figure 4.

Quantitative analyses of various cellular markers by immunocytochemistry (A–B) and RT-PCR (C). Notably, the percentage of cells expressing Nestin decreased from 99% to 7% during in vitro differentiation, because Nestin is preferentially expressed in undifferentiated neural progenitors. However, the expression of MAP2 and β3-tubulin conversely increased over time. Legend: x-axis – days in differentiation conditions; y-axis – number of counted cells (A), % of counted cells (B), and relative expression in RNA (C). Reproduced with permission from [27].