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Western blotting to detect MAPK activity: AAV293 packaging cells were collected 24 h after a triple or quadruple transfection (+miR‐431 or miR‐636) for generation of AAV2‐EGFP vectors. The protein lysates were resolved by SDS‐PAGE analysis and probed for p44/42 MAPK proteins. β‐actin was used as a loading control. Data are representative of three independent experiments
