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. 2020 Mar 3;412(12):2815–2827. doi: 10.1007/s00216-020-02508-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Comparison of peanut allergen determination in spike plasma by ELISA (a) and Q-TOF-MRM (b). ELISA analysis of the peanut extract was used to calculate the percentage of each allergen in the extract. Calculated values were used to estimate the amount of allergen in each spiked samples assuming no losses during processing. This was compared with the amount of allergen detected in the spiked serum samples using the same ELISA kits (a) or the Q-TOF-MRM method (b)