Table 1.

Acetylation-sensitive proteins in autophagy

Protein	Function in autophagy	Acetylation sensitive Lys residues	Acetylase	Deacetylase	Outcome of deacetylation
TFEB	Transcription factor	(Lys91), (Lys103), Lys116, Lys274, Lys279 and (Lys430)	ACAT1? GCN5	SIRT1 HDAC2/6	Increased lysosomal biogenesis, transcription of ATG proteins
FoxO1	Transcription factor	Lys242, Lys245, Lys262 (mouse residues)	CBP,p300	SIRT1	Increased DNA binding
FoxO3a	Transcription factor	Unknown	Unknown	SIRT1-3	Cytoplasm to nucleus translocation
ULK1	ULK1 complex member	Lys162 and Lys606	TIP60	Unknown	Loss of kinase activity stimulation
VPS34	Class III PI3K kinase complex member	Lys29, Lys771, (Lys781)	p300	Unknown	Increased complex formation (Lys29), increased PI binding (Lys771)
Beclin 1	Class III PI3K kinase complex member	Lys430 and Lys437	p300	SIRT1	Autophagosome maturation
ATG3	Autophagosome elongation	Lys19, Lys48, Lys183 (yeast residues)	TIP60	HDAC1/2	Decreased membrane-binding
ATG7	Autophagosome elongation	Unknown	p300	SIRT1	Increased LC3-PE formation
ATG5	LC3–PE deconjugation	Unknown	p300	SIRT1 SIRT2 SIRT3	Increased LC3-PE formation
ATG12	LC3–PE deconjugation	Unknown	p300	Unknown	Increased LC3-PE formation
LC3	Multiple	Lys49 and Lys51	p300	SIRT1	Increased levels of LC3-PE formation
Ub	Selectivity	Lys6 and Lys48	Unknown	Unknown	Poly-Ub chain formation
p300	Inhibits autophagy	Multiple	p300	SIRT2	Loss of inhibitory ATG5/ATG7/ATG12/LC3 acetylation
p62	Selective cargo recognition	Lys420 and Lys435	TIP60	HDAC6	Increased Ub binding

() lysine residues sensitive to acetylation, but their involvement in autophagy regulation remains unknown