Figure 6.

Upregulation of SIRT1 was involved in the neuroprotective effects of KFL against OGD in PC12 cells. (a) Cell lysates were extracted and subjected to immunoprecipitation with an anti-P66shc antibody. The precipitates were then evaluated with an antiacetylated-lysine (Ac-lysine) antibody. Protein expression of SIRT1 was determined using western blot. (b) The efficiency of LV-shSIRT1 transfection was confirmed by real-time PCR. (c) ROS level was measured using an oxidant sensitive probe. (d) Apoptosis was measured using a TUNEL assay kit. (e) LDH release in medium was determined using a commercial kit. (f) Cell viability was determined using the CCK8 assay kit. ^∗p < 0.05.