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. 2020 Apr 28;13:3535–3544. doi: 10.2147/OTT.S249816

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© 2020 Deng et al.

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Characterization of VG9-GMCSF-IL24. (A) Schematic illustration of VG9-GMCSF-IL24 construction. GM-CSF and IL-24 co-expression cassettes linked by F2A were inserted into TK locus of VG9 strain via homologous recombination. (B) Western blotting analysis of GM-CSF and IL-24 proteins from Vero cells treated with PBS, or VG9-EFGP, VG9-GM-CSF, VG9-IL-24, VG9-GMCSF-IL24 at 0.1 MOI for 48 h. (C) Supernatants and lysates from various cell lines treated with VG9-GM-CSF or VG9-GMCSF-IL24 were collected and GM-CSF concentrations were quantified by ELISA. (D) Supernatants and lysates from various cell lines treated with VG9-IL-24 or VG9-GMCSF-IL24 were collected and IL-24 concentrations were quantified by ELISA. Each bar represents the mean ± SD (n=3).