Table 1.

All Eligible Studies Were Categorized Based on Methods of Isolation of EVs, Clinical Applications and Target Parasites

No.	Author	Year	Method of Isolation	Applications	Parasite
1	Roig40	2018	Ultracentrifugation 120,000 × g for 1h at 4ºC	Immunotherapy	F. hepatica
2	Shears41	2018	Ultracentrifugation 100,000 × g for 2 h at 4ºC	Vaccination	T. muris
3	Eichenberger42	2018	Ultracentrifugation 120,000 × g for 3h at 4ºC	Immunotherapy	N. brasiliensis & T. muris
4	Wang43	2018	Ultracentrifugation 105,000 × g for 1.5h at 4ºC	Diagnosis	A. suum
5	Bautista-López44	2017	Ultracentrifugation 100,000 × g for 16h at 4°C	Diagnosis	T. cruzi
6	Li45	2018	ExoEasy Maxi Kit	Vaccination	T. gondii
7	Coakley46	2017	Ultracentrifugation 100,000 × g for 2 h at 4ºC	Vaccination	H. polygyrus
8	del Cacho47	2011	Ultracentrifugation 100,000 × g for 1 h at 4ºC	Vaccination	E. tenella
9	Meningher48	2017	Ultracentrifugation 100,000 × g at 4ºC	Diagnosis	Schistosoma spp.
10	Trelis49	2016	Ultracentrifugation 120,000 × g for 1 h at 4ºC	Immunotherapy	E. caproni
11	Olmos-Ortiz50	2017	Ultracentrifugation 100,000 × g for 2 h	Immunotherapy	T. vaginalis
12	Martin-Jaular51	2011	Ultracentrifugation 100,000 × g for 2 h at 4ºC	Vaccination	P. yoelii
13	Buck52	2014	Ultracentrifugation 100,000 × g for 2 h at 4ºC	Immunotherapy	H. polygyrus
14	Schnitzer53	2010	Ultracentrifugation 100,000 × g for 5 h	Vaccination	L. major
15	del Cacho54	2012	Ultracentrifugation 100,000 × g for 1 h at 4ºC	Vaccination	E. tenella, E. maxima, and E. acervulina
16	del Cacho55	2016	Total Exosome Isolation Reagent (from serum)	Vaccination	E. tenella
17	Antwi-Baffour56	2019	Ultracentrifugation 100,000 × g for 90 min	Diagnosis	P. falciparum

Abbreviation: EVs, extracellular vesicles.