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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: Acta Neuropathol. 2020 Jan 3;139(3):565–582. doi: 10.1007/s00401-019-02117-6

Figure 6. Mutations in human POGLUT1 reduce its myogenic activity in transgenic flies.

Figure 6.

a-b Staining of Drosophila indirect flight muscles at 25% pupal development in y w (control) and rumi79/79 animals using α−22C10 antibody (red); c-h staining of Drosophila indirect flight muscles at 25% pupal development in rumi79/79 animals upon Mef2-GAL4 driven overexpression of FLAG-tagged WT and indicated mutant human POGLUT1 with α-FLAG antibody (red). All animals were raised at 25°C. Scale bar, 200 μm. i Graph shows the quantification to myotube length in the indicated genotypes. Horizontal bars represent Mean ± SD. Average length of 3–6 myotubes per animal from 3–4 animals per genotype was scored. Significance is ascribed as *P<0.05 and ****P<0.0001 compared to rumi79/79, and $ $ $P<0.001 and $ $ $ $P<0.0001 compared to y w. One-way ANOVA with Dunnett’s multiple comparisons test was used to determine the P-values (Supplementary Source Data 3, online resource).