Table 1.
List of Primers Used in This Study
| Name of Gene | Primer Sequence | Amplicon Size | PCR Condition | Reference |
|---|---|---|---|---|
| Colicin V | F:CACACACAAACGGGAGCTGTT R:CTTCCCGCAGCATAGTTCCAT |
680 bp | 35 cycles of 94 ºC for 30s, 55 ºC for 30 s, 72 ºC for 1 min | (Johnson and Stell, 2000) [35] |
| Colicin Ia/Ib | F:ACGTATTACAAATCCCGGTGC R:CTTTTTCCTTCAACAGGGCA |
1250 bp | 35 cycles of 94 ºC for 30s, 55 ºC for 30 s, 72 ºC for 1 min | (Schamberger and Diez-Gonzalez, 2004) [36] |
| Colicin B | F:AAGAAAATGACGAGAAGACG R:GAAAGACCAAAGGCTATAAGG |
493 bp | 35 cycles of 94 ºC for 30s, 55 ºC for 30 s, 72 ºC for 1 min | (Schamberger and Diez-Gonzalez, 2004) [36] |
| Colicin M | F:CATCACCATCAACTAACTTACC R:CTCTTTACCAGAAAACATCG |
737 bp | 35 cycles of 94 ºC for 30s, 55 ºC for 30 s, 72 ºC for 1 min | (Schamberger and Diez-Gonzalez, 2004) [36] |
| Colicin E1 | F:TTTGAATGGTACTCCTGACGG R:GTTCCAGCAAGCAAGCTAAA |
1398 bp | 35 cycles of 94 ºC for 30s, 55 ºC for 30 s, 72 ºC for 1 min | (Schamberger and Diez-Gonzalez, 2004) [36] |
| Colicin E2-E9 | F:CGACAGGCTAAAGCTGTTCAGGT R:TGCAGCAGCATCAAATGCAGCCT |
219 bp | 35 cycles of 94 ºC for 1 min, 60 ºC for 1 min, 72 ºC for 1 min | (Tahamtan et al, 2012) [40] |
| crl | F:TTTCGATTGTCTGGCTGTAT R:CTTCAGATTCAGCGTCGTC |
250 bp | 30 cycles of 94 ºC for 40 s, 52ºC for 40 s, 72ºC for 45 s | (Pal and Singh, 2007) [12] |
| csgA | F:ACTCTGACTTGACTATTACC R:AGATGCAGTCTGGTCAAC |
200 bp | 30 cycles of 94 ºC for 1 min, 48ºC for 1min, 72ºC for 1 min | (Pal and Singh, 2007) [12] |
| fimH | F:TGCAGAACGGATAAGCCGTGG R:GCAGTCACCTGCCCTCCGGTA |
508 bp | 25 cycles of 94ºC for 30s, 63 ºC for 30 s, 68 ºC for 3min | (Johnson and Stell, 2000) [35] |
| hlyA | F:AACAAGGATAAGCACTGTTCTGGCT R:ACCATATAAGCGGTCATTCCCGTCA |
1177 bp | 25 cycles of 94ºC for 30s, 63 ºC for 30 s, 68 ºC for 3min | (Yamamoto et al, 1995) [34] |
| traT | F:GGTGTGGTGCGATGAGCACAG R:CACGGTTCAGCCATCCCTGAG |
290 bp | 25 cycles of 94ºC for 30s, 63 ºC for 30 s, 68 ºC for 3min | (Johnson and Stell, 2000) [35] |
| bla−OXA-2 | F: AAGAA ACGCTACTCGCCTGC R: CCACTCAACCCATC CTACCC′ |
478 bp | 40 cycles of 94 ºC for 1 min, 55ºC for 1min, 72ºC for 1 min | (Bhattacharjee et al, 2007) [38] |
| bla−OXA-10 | F:TCTTTCGAGTACGGCATTAGC R:CCA ATGATGCCCTCACTTTCC |
760bp | 35 cycles of 96 ºC for 1 min, 56ºC for 1min, 72ºC for 1 min | (Lin et al, 2012) [39] |
| bla−CTX-M-5 | F:CGCTTTGCGATGTGCAG R:ACCGCGATATCGTTGGT |
550 bp | 40 cycles of 94 ºC for 1 min, 55ºC for 1min, 72ºC for 1 min | (Bhattacharjee et al, 2007) [38] |