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. 2020 Apr 6;11(14):4059–4072. doi: 10.7150/jca.44622

Figure 1.

Figure 1

CG-745 shows more potent HDAC inhibition compared to other HDACis and induces cell cycle arrest and cell death relatively early: (A) In vitro histone deacetylase inhibitory activity of CG (CG-745), Vor (vorinostat), Ent (entinostat), and Res (resminostat). HDAC activity was analyzed at different HDAC inhibitor concentrations by measuring HDAC substrate fluorescence. Results are shown as means based on experiments performed in triplicate (B) Cytotoxicity of each HDACis on Hep3B cells was analyzed with MTS following 72 hours post-exposure incubation (*p< 0.05); (C, D) Cells were stained by Annexin-V and PI (C), or PI only (D) following 24 hours post-exposure incubation, and samples were analyzed by Attune NxT (Invitrogen) for cell death and cell cycle; (E) Hep3B cells were incubated for 24 hours with HDACis and the total extract was analyzed for expression or phosphorylation of indicated proteins on a text by Western blot.