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Primer sequence for real-time PCR
| Gene | Forward primer (5′- to 3′) | Reverse primer (5′- to 3′) |
|---|---|---|
| EphB1 | ATGCGCTTCACTGTGAGAGAC | ATTCCGAGTAAGAGGCCCAAA |
| CDH2 | AGCCAACCTTAACTGAGGAGT | GGCAAGTTGATTGGAGGGATG |
| Snail | TCAAGATGCACATCCGAAGCC | TTGTGGAGCAGGGACATTCG |
| Zeb1 | GCACAACCAAGTGCAGAAGA | GCCTGGTTCAGGAGAAGATG |
| Slug | TGGTCAAGAAACATTTCAACGCC | GGTGAGGATCTCTGGTTTTGGTA |
| Smad2 | CCGACACACCGAGATCCTAAC | GAGGTGGCGTTTCTGGAATATAA |
| CDH1 | TGAAGCCCCCATCTTTGTGC | GGCTGTGTACGTGCTGTTCT |
| GAPDH | AACGGATTTGGTCGTATTGG | TTGATTTTGGAGGGATCTCG |
