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. 2020 Apr 6;11(14):4037–4046. doi: 10.7150/jca.30360

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Effect of CXCR4 silencing on various phenotypes of the PC9 cell line. Low levels of CXCR4 expression were compared with higher levels of CXCR4 expression in: (A) soft agar colony formation assays, (B) wound healing assays, (C) transwell assays, and (D) CCK8 assays. These assays were performed to evaluate colony formation, cell migration, cell invasion and cell viability, for the PC9 cell groups indicated, respectively. In panel C, crystal violet stained cells (at left) and the corresponding quantitation of these stained cells (at right) indicate the number of invasion cells for each group. PC9-KD: PC9/CXCR4-shRNA1 group; PC9-C: PC9/shCtrl group; PC9: untransfected PC9 group.