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. 2020 Apr 27;10(12):5623–5640. doi: 10.7150/thno.44836

Figure 1.

Figure 1

BCAA promote FAO in cardiac myocytes. (A) Adult mouse ventricular myocytes were isolated and treated with different concentrations of BCAA (0, 0.429 mM, 0.858 mM, 1.716 mM, 3.432 mM) for 12 h. Expression of Cpt1b, Acsl, Acaa2, Acadm, Slc27a1, Slc27a6, Cd36, Fabp3 mRNA by real-time PCR, normalized to β-actin (n=6). (B-G) Adult mouse cardiac myocytes were treated with or without BCAA (3.432 mM) for 12 h. FAO levels were determined by seahorse analyzer (n=4-5). (B) OCR curve of Con (No BCAA) group and BCAA group were determined. (C) Basal respiration (D) ATP production (E) maximal respiration (F) basal respiration due to exogenous palmitate-BSA and (G) maximal respiration due to exogenous palmitate-BSA were calculated according to instruction. (C-E) Data were analyzed by one-way ANOVA, followed by a Bonferroni post-hoc test. (F-G) Data were analyzed by Student's t test (two-tailed). * P<0.05, ** P<0.01. All values are presented as mean ± SEM.