Figure 2.

BCAA exacerbate H/R injury via enhancing FAO. NRVMs were isolated and subjected to 9 h/3 h H/R, with or without BCAA (3.432 mM) and Eto (10 nmol/L). (A) Cleavage of caspase-3 and total caspase-3 were determined by western blotting (n=6). (B) Apoptosis was analyzed by Annexin V-FITC flow cytometry (n=6). (C-E) LDH release, MDA and 4-HNE were determined as methods described (n=6). (F) Superoxide generation was assessed by DHE staining (n=6). Data were analyzed by one-way ANOVA, followed by a Bonferroni post-hoc test. * P<0.05, ** P<0.01.