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. 2020 Apr 6;10(12):5290–5304. doi: 10.7150/thno.42658

Figure 4.

Figure 4

PTPROt deficiency suppresses ROS production leading to decreased activation of the NLRP3-caspase-1 axis. A. NLRP3, NLRP1, NLRC4, and AIM2 in cell homogenates from primary liver macrophages isolated from the mice described in Figure 1A. B. NLRP3, NLRP1, NLRC4, and AIM2 in cell homogenates from primary liver macrophages isolated from the mice described in Figure 1B. C. Immunoblotting of NLRP3, pro-caspase-1, caspase-1-p22, caspase-1-p20, and β-actin (loading control) in primary liver macrophages isolated from the mice described in Figure 1A, B. D. NLRP3, NLRP1, NLRC4, and AIM2 in cell culture supernatants from RAW-ctrl and RAW-PTPROt+ cells treated with free fatty acids (FFAs), with or without pyrrolidine dithiocarbamate (PDTC) for 24 hr. E. Immunoblotting of NLRP3, pro-caspase-1, caspase-1-p22, caspase-1-p20, and β-actin (loading control) in RAW-PTPROt+ cell lines treated with FFAs, with or without PDTC for 24 hr. Abbreviations: AIM2: Absent In Melanoma 2; MCD: methionine-choline-deficient diet; FFAs: Free fatty acids; NLRC4: NLR family CARD domain-containing protein 4; NLRP1: NLR family pyrin domain containing 1; NLRP3: NLR family pyrin domain containing 3; KO: the primary liver macrophages isolated from PTPROt knockout mice; PDTC: Pyrrolidine dithiocarbamate; WD: Western diet; WT: the primary liver macrophages isolated from wild-type mice.