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. 2020 Apr 6;10(12):5225–5241. doi: 10.7150/thno.43716

Figure 6.

Figure 6

PTL treatment altered the cytokine profile in colitis mice. (A) Volcano plot of cytokine alteration from colon tissue supernatant as measured by Luminex bead-based immunoassays. (B) Heatmap representation of cytokine levels from colon tissue supernatant as measured by Luminex (PTL+DSS group, n = 8; DSS group, n = 6). Significance analysis of microarrays (SAM) was used to identify tissue supernatant proteins at significantly different levels between groups (q < 0.001, fold-change > 2). A hierarchically clustered (unsupervised, Euclidean distance) heatmap of the SAM-positive proteins is shown. The raw mean fluorescence intensity (MFI) of all proteins were log2 transformed prior to clustering. (C) IL-1β, IL-6, TNF-α, IL-10 and IL-17A cytokines levels in colon tissue homogenate were measured by ELISA between PTL+DSS and DSS groups. (D) IL-1β, IL-6, TNF-α, IL-10 and IL-17A cytokines levels in colon tissue homogenate were measured by ELISA between ABX(PTL+DSS) and ABX(DSS) groups. (E) IL-1β, IL-6, TNF-α, IL-10 and IL-17A cytokines levels in colon tissue homogenate were measured by ELISA between FM(PTL+DSS) and FM(DSS) groups. (C-E) n = 5 mice per group, mean values ± SD are presented, p values were calculated using Unpaired T-test, * p < 0.05, ** p < 0.01, *** p < 0.001. Data are pooled from three independent experiments with n = 5 mice per group.