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Subcellular localization of CDYL2a and CDYL2b. (A-D) MDA-MB-231 and Hs578T cells were transfected with plasmid DNAs encoding empty vector, pCDH, Flag-CDYL2a, or Flag-CDYL2b. After 48 h of transfection, cells were subjected to immunofluorescence staining with an antibody against Flag (red), H3K9me3 (A), heterochromatin protein 1α (HP1α) (B), SC35 (C), or fibrillarin (FBL) (D). DNA was counterstained with DAPI (blue).
