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CRISPR/Cas9 Delivery Formats
| Format | Time to Cas9 Onset Expression | Advantages | Disadvantages |
|---|---|---|---|
| pDNA | 5 hours | Low cost of production Prolonged expression of CRISPR components |
High possibility of insertional mutagenesis High risk of off-target effects |
| mRNA | 1 hour | No insertional mutagenesis Fast expression of CRISPR components |
High cost of production |
| RNP | Immediately | High editing efficiency Low risk of off-target effects |
High cost of production |
