Table 3.
Transition State Thermodynamics for the Intrinsic GTPase Activity of K-RAS and Its Oncogenic Mutantsa
| protein | ΔH‡ (kcal/mol) | ΔS‡ (kcal/mol) | ΔG‡ (kcal/mol) | ΔΔS‡ (kcal/mol) | ΔΔH‡ (kcal/mol) |
|---|---|---|---|---|---|
| WT | 17.0 ± 0.3 | −0.0133 ± 0.0011 | 20.9 ± 0.01 | ||
| G12C | 19.3 ± 0.1 | −0.0084 ± 0.0005 | 21.8 ± 0.01 | 0.0049 ± 0.0012 | 2.3 ± 0.4 |
| G13D | 18.0 ± 0.5 | −0.0119 ± 0.0018 | 21.6 ± 0.02 | 0.0014 ± 0.0021 | 1.0 ± 0.6 |
| Q61H | 19.6 ± 0.5 | −0.0076 ± 0.0014 | 22.3 ± 0.02 | 0.0058 ± 0.0018 | 2.6 ± 0.6 |
a
ΔG‡ is calculated directly from enthalpy and entropy at 25 °C. Reported are the mean and standard deviation of three replicates (n = 3).